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RCPB Carboxypeptidase B Lyophilized Powder Animal Origin Free

RCPB01

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Description

RCPB Carboxypeptidase B Lyophilized Powder Animal Origin Free

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Description ? Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 33.8kD. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis. Source:E. coli Species:Rat Form:Lyophilized from 20 mM Tris, pH 8.0 + 50 mM NaCl. Enzyme Commission Number: 3.4.17.2 ?
Main Features

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Source | E. Coli |
Purified by | HPLC |
Format | White or white-like?or yellowish?lyophilized |
Additives | Tris salts, NaCl salts and carbohydrates |
Protein content | 35% ～ 60% |
Purity | Single main band on SDS-PAGE |
M.W. | 33.8kD |
Contaminant activity | Less than 10 ppm of recombinant trypsin. |
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Unit Definition

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One Unit of carboxypeptidase B activity hydrolyzes one micromole of?hippuryl-L-arginine per minute at 25℃, pH 7.65.

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Recommend Usage

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To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH?7-9.

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Storage Instructions

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Recommend recombinant carboxypeptidase B lyophilized?should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90%?activity remained?after 10 times repeated freezing and thawing.



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?Email:Zhaozhi@yaxinbio.com

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